新型脱氧核酶ASON-Bulge-DNAzyme靶向M蛋白抑制PRRSV在MARC-145细胞中的复制

doi:10.11843/j.issn.0366-6964.2014.12.013

Abstract

Abstract:

This study aimed to explore，on the cell level，the possibility of using ASON-Bugle-DNAzyme，as a new genetherapy in inhibiting reproductive and respiratory syndrome virus （PRRSV）.Two novel DNAzymes targeting the PRRSV M gene were designed.MARC-145 cells were infected with PRRSV 10 h after the active ASON-Bulge-DNAzymes were delivered，efficiency of ASON-Bulge-DNAzymes were assayed by RT-PCR，TCID₅₀，CPE and indirect immunofluorescence assay (IFA).The experiment successfully derived the active ASON-Bulge-DNAzyme inhibition of PRRSV replication procedure in MARC-145 cells and showed that the two interference target sites may be necessary for PRRSV replication.The outcomes from this study will increase our knowledge and lead to the development of DNAzyme in virus diseases control.The results may be used for future study of PRRSV replication，antiviral therapy and prevention of PRRSV.

CLC Number:

S852.659.6

LI Hong，LIU Cheng-qian，GAO Jun，YI Jian-zhong. Inhibition of Porcine Reproductive and Respiratory Syndrome Virus Replication by M-ASON-Bulge-DNAzyme in MARC-145 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2000-2005.

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Inhibition of Porcine Reproductive and Respiratory Syndrome Virus Replication by M-ASON-Bulge-DNAzyme in MARC-145 Cells

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